Effect of dexmedetomidine on AKT/ERK signaling pathway and EMT-related proteins in high glucose-induced apoptosis in human renal tubular epithelial cells


 Purpose: To study the effect of dexmedetomidine (Dex) on AKTERK signaling pathway and EMT- related proteins in high glucose-induced apoptosis human kidney tubular epithelial cells.
 Methods: HK-2 cells were assigned to control, high-glucose Dex groups. Levels ROS determined using live cell station. Flow cytometry was used measure cycle while Western blot applied assay levels PI3K, Akt, p-Akt, ERK p-ERK.
 Results: The concentrations markedly reduced group, relative glucose group (p < 0.05). Apoptosis P13K protein significantly lower groups than their corresponding control levels. In glucose-treated cells, AKT expression downregulated, p-AKT Protein expressions p-ERK values, but accentuated 0.05).
 Conclusion: mitigates increases proportion G1 phase, reduces EMT via a mechanism regulation pathway-associated proteins. provide insights into development drugs for treatment diabetic nephropathy.